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ATCC
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ATCC
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ATCC
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ATCC
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ATCC
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ATCC
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DSMZ
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DSMZ
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Cyagen Biosciences
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Promega
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Cyagen Biosciences
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Addgene inc
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Image Search Results
Journal: Cell
Article Title: Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design.
doi: 10.1016/j.cell.2018.10.037
Figure Lengend Snippet: Figure 5. Semi-synthetic Synthesis and Antibacterial Effects of TUN-X,X Analogues (A) Semi-synthetic strategy for TUN mimics. (B–D) MIC obtained from micro-broth dilution antimicrobial susceptibility tests of (B) B. subtilis EC1524, (C) B. cereus ATCC11778, (D) Mtb H37Rv (ATCC27294) cultured in 7H9/ADC/Tw (black), or GAST/Fe (grey) media (n=3). For all panels data presented are means ± SD
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains MAX Efficiency DH10Bac Thermo Fisher Cat# 10361012 Biological Samples Mouse liver microsomes Sigma Cat# M9441 Human liver microsomes Sigma Cat# M 0317 Chemicals, Peptides, and Recombinant Proteins Tunicamycin Sigma-Aldrich Cat# T7765; CAS: 11089-65-9 uridine diphosphate n-acetyl [1-14C] D-glucosamine American Radiolabeled Chemicals Cat# ARC 0151 Rifampicin Sigma-Aldrich Cat# R3501 Uridine 50-diphospho-N-acetylglucosamine sodium salt Sigma-Aldrich Cat# U4375 C95-Dolichyl-MPDA Larodan Cat# 67-1095 H. sapiens DPAGT1 (1-408, N-terminal 6 x HIS tag, TEV cleavage site) This work N/A H. sapiens DPAGT1 (1-408, Val264Gly, N-terminal 6 x HIS tag, TEV cleavage site) This work N/A Critical Commercial Assays MemGold2 HT-96 screen Molecular Dimensions Cat# MD1-64 Deposited Data H. sapiens DPAGT1 (WT) This work PDB: 6FM9 H. sapiens DPAGT1 (Val264Gly) This work PDB: 5LEV H. sapiens DPAGT1 (Val264Gly) + UDP-GlcNAc + Mg2+ This work PDB: 6FWZ H. sapiens DPAGT1 (Val264Gly) + Tunicamycin This work PDB: 5O5E Experimental Models: Cell Lines Spodoptera frugiperda (Sf9) insect cells Thermo Fisher Cat# 11496015; RRID:CVCL_0549 HepG2 ATCC Cat# ATCC HB-8065 HEK293 ATCC Cat# ATCC CRL-1573; RRID:CVCL_0045 Raji ATCC Cat# ATCC CCL-86; RRID:CVCL_0511 J774A.1 ATCC Cat# ATCC TIB-67; RRID:CVCL_0358 Experimental Models: Organisms/Strains Streptomyces chartreusis DSMZ Cat# 41447 Bacillus subtilis EC1524 John Innes Centre N/A Micrococcus luteus John Innes Centre N/A Bacillus cereus DSMZ Cat# 345 Escherichia coli Thermo Fisher Cat# ATCC 25922 Staphylococcus aureus Thermo Fisher Cat# ATCC 29219 Pseudomonas aeruginosa Thermo Fisher Cat# ATCC
Techniques: Analogues, Cell Culture