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ATCC cat crl 3022 hek293 atcc cat crl 1573 sf9 gibco cat 12659017 b
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Addgene inc 27853 mycobacterium tuberculosis h37rv atcc
Figure 5. Semi-synthetic Synthesis and Antibacterial Effects of TUN-X,X Analogues (A) Semi-synthetic strategy for TUN mimics. (B–D) MIC obtained from micro-broth dilution antimicrobial susceptibility tests of (B) B. subtilis EC1524, (C) B. cereus ATCC11778, (D) Mtb <t>H37Rv</t> (ATCC27294) cultured in 7H9/ADC/Tw (black), or GAST/Fe (grey) media (n=3). For all panels data presented are means ± SD
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Figure 5. Semi-synthetic Synthesis and Antibacterial Effects of TUN-X,X Analogues (A) Semi-synthetic strategy for TUN mimics. (B–D) MIC obtained from micro-broth dilution antimicrobial susceptibility tests of (B) B. subtilis EC1524, (C) B. cereus ATCC11778, (D) Mtb H37Rv (ATCC27294) cultured in 7H9/ADC/Tw (black), or GAST/Fe (grey) media (n=3). For all panels data presented are means ± SD

Journal: Cell

Article Title: Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design.

doi: 10.1016/j.cell.2018.10.037

Figure Lengend Snippet: Figure 5. Semi-synthetic Synthesis and Antibacterial Effects of TUN-X,X Analogues (A) Semi-synthetic strategy for TUN mimics. (B–D) MIC obtained from micro-broth dilution antimicrobial susceptibility tests of (B) B. subtilis EC1524, (C) B. cereus ATCC11778, (D) Mtb H37Rv (ATCC27294) cultured in 7H9/ADC/Tw (black), or GAST/Fe (grey) media (n=3). For all panels data presented are means ± SD

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains MAX Efficiency DH10Bac Thermo Fisher Cat# 10361012 Biological Samples Mouse liver microsomes Sigma Cat# M9441 Human liver microsomes Sigma Cat# M 0317 Chemicals, Peptides, and Recombinant Proteins Tunicamycin Sigma-Aldrich Cat# T7765; CAS: 11089-65-9 uridine diphosphate n-acetyl [1-14C] D-glucosamine American Radiolabeled Chemicals Cat# ARC 0151 Rifampicin Sigma-Aldrich Cat# R3501 Uridine 50-diphospho-N-acetylglucosamine sodium salt Sigma-Aldrich Cat# U4375 C95-Dolichyl-MPDA Larodan Cat# 67-1095 H. sapiens DPAGT1 (1-408, N-terminal 6 x HIS tag, TEV cleavage site) This work N/A H. sapiens DPAGT1 (1-408, Val264Gly, N-terminal 6 x HIS tag, TEV cleavage site) This work N/A Critical Commercial Assays MemGold2 HT-96 screen Molecular Dimensions Cat# MD1-64 Deposited Data H. sapiens DPAGT1 (WT) This work PDB: 6FM9 H. sapiens DPAGT1 (Val264Gly) This work PDB: 5LEV H. sapiens DPAGT1 (Val264Gly) + UDP-GlcNAc + Mg2+ This work PDB: 6FWZ H. sapiens DPAGT1 (Val264Gly) + Tunicamycin This work PDB: 5O5E Experimental Models: Cell Lines Spodoptera frugiperda (Sf9) insect cells Thermo Fisher Cat# 11496015; RRID:CVCL_0549 HepG2 ATCC Cat# ATCC HB-8065 HEK293 ATCC Cat# ATCC CRL-1573; RRID:CVCL_0045 Raji ATCC Cat# ATCC CCL-86; RRID:CVCL_0511 J774A.1 ATCC Cat# ATCC TIB-67; RRID:CVCL_0358 Experimental Models: Organisms/Strains Streptomyces chartreusis DSMZ Cat# 41447 Bacillus subtilis EC1524 John Innes Centre N/A Micrococcus luteus John Innes Centre N/A Bacillus cereus DSMZ Cat# 345 Escherichia coli Thermo Fisher Cat# ATCC 25922 Staphylococcus aureus Thermo Fisher Cat# ATCC 29219 Pseudomonas aeruginosa Thermo Fisher Cat# ATCC 27853 Mycobacterium tuberculosis H37Rv ATCC Cat# ATCC27294 C57Bl/6 mice Taconic B6 Oligonucleotides Oligonucleotides See table S8 B6 Recombinant DNA pFB-LIC-Bse This paper N/A DPAGT1 gene Source BioScience LifeSciences IMAGE:2821845 pMSP12::GFP Addgene Cat# 30167 (Continued on next page) Cell 175, 1045–1058.e1–e9, November 1, 2018 e1

Techniques: Analogues, Cell Culture